ZEISS at FOM 2014

The moment your data change
scientific minds.

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ZEISS at FOM 2014

April 13 - 16, Booth #6 and #7 - Sydney, Australia

Visit our booth and discover ZEISS systems for light sheet fluorescence microscopy (LSFM), superresolution and correlative microscopy.

 

ZEISS Lightsheet Z.1

See how Lightsheet Z.1 performs fast fluorescence imaging on large, living samples; with virtually no phototoxicity or bleaching and with excellent temporal resolution.




Learn more about Lightsheet Z.1

ZEISS ELYRA PS.1

Explore the exciting world of superresolution. Learn how ELYRA performs supperresolution structured illumination microscopy (SR-SIM) and photoactivated localization microscopy in three dimensions (3D-PALM).


Learn more about ELYRA PS.1

Correlative Microscopy

Bridge the gap between micro and nano worlds. ZEISS Shuttle & Find is a combined hardware and software solution that connects widefield, laser scanning and superresolution microscopes with all ZEISS focused ion beam (FIB-SEM) and scanning electron microscopes (SEM).

Learn more about correlative microscopy

  • Lightsheet Z.1

    Lightsheet Z.1 Presentation

    Our application specialist Dr. Daniel Koch will host a presentation discussing the advantages and applications of light sheet fluorescence microscopy.
    Presentations will be held on April 14th and 15th between 2 and 3 pm at Lecture Theatre 123.
    Please email us to register your interest.

     

    Lightsheet Z.1 Hands-on Workshop

    Take part in a hands-on Lightsheet Z.1 workshop. Discover the advantages of light sheet fluorescence microscopy (LSFM). Learn how to collect amazing time-lapse fluorescent images of the development of your large living samples and get insights on this exciting new technology.
    Please register at the ZEISS booth #6 and #7 - York Foyer.

     

     

  • 3D Superresolution Microscopy

    “Superresolution Microscopy in 3D” – Photoactivated Localization and Structured Illumination Microscopy to study cellular structures and dynamics

    Resolving ultrafine details of subcellular structures is key to understand the organization and functioning of cellular networks. Recent advances in far-field fluorescence microscopy provide the necessary tools to analyze these structures with resolutions well below the classical diffraction limit in all three-dimensions. These technical advances went hand in hand with improved versions of photo-switchable fluorophores that allowed to push resolution limits further down. The wide spectrum of suitable dyes along with the high contrast achieved endow these fluorescence based superresolution (SR) techniques with the power to study the complexity of sub-cellular organelles and the relation of their constituting components down to the molecular level and under physiological conditions. They provide us in this way with a far better understanding of the assembly of macromolecular complexes and their functions within a cell than has been possible before employing conventional imaging methods. Here we will give an overview of the technical state-of-the art of two of these technologies, Structured Illumination Microscopy (SIM) and Photo-activated Localization Microscopy (PALM), and provide typical application examples in this exciting field. We will particularly focus on the 3D capability of both methods.

    Speaker
    Klaus Weisshart, Carl Zeiss Microscopy GmbH, Jena, Germany

    Date
    April 14, 2014 at 5:30 pm

    Location
    University of Sydney,
    Seymour Centre - The York Theatre,
    Corner of City Rd and Cleveland St,
    Chippendale NSW 2008

  • CLEM Presentation

    Correlative Light and Electron Microscopy - on the way from 2D towards 3D

    Correlative microscopy bridges the gap between light and electron microscopy. The previously introduced “Shuttle & Find” interface is the first easy to use solution for imaging one and the same sample regions in different microscope systems. It allows the straightforward relocation of an area of interest which was investigated before in a different microscope system. The next step will be to address correlative 3D applications. Two approaches for the generation of 3D correlative data sets are of major interest: Correlative array tomography and Correlative 3D LM-FIB.
    Correlative array tomography allows the detection of fluorescent labels as well as ultrastructural investigations on ultrathin serial sections. Regions of interest can be marked and automatically imaged within all the individual sections building up long ribbons. The challenge of this approach is on one hand the alignment of the consecutive 2D images taken on light microscope and electron microscope and on the other hand their subsequent registration to a correlative 3-dimensional data set.
    While correlative array tomography is sample preserving it can only be performed on fixed and sliced specimen. For the correlation of life cell imaging with highly resolved ultrastructure the use of optical sectioning methods and FIB is the combination of choice. This allows to analyse large volume dynamics and correlate these data with the ultrastructural information semi-automatically. The combination of 2-Photon microscopy and FIB-SEM avoids the typical problems which are normally encountered with serial tomography. Crossbeam systems provide 3D datasets of comparatively large volumes that can be analysed with maximum precision and high spatial resolution. Time consuming alignment procedures generally used in tomography are not needed, simple cross correlation methods are sufficient to achieve precise alignment of data obtained.

    Speaker
    Christian Boeker, Carl Zeiss Microscopy GmbH, Germany

    Date
    April 15, 2014 at 2:00 pm

    Location
    University of Sydney,
    Seymour Centre - Studio 1,
    Corner of City Rd and Cleveland St,
    Chippendale NSW 2008

  • CLEM Workshop

    CLEM Workshop - Ultrastructure Lights Up

    Please join us for four talks exploring the latest developments in correlative microscopy. The Correlative Light Electron Microscopy (CLEM) Masterclass will also incorporate the 4th Australian CLEM Workshop.

    CLEM Tutorial Announcement

    CLEM Abstract Tutorial

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